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Old 07-02-2012, 11:01 AM   #1
HMorrison
Senior Member
 
Location: Massachusetts

Join Date: May 2009
Posts: 116
Default 96 well PCR clean up

Anyone found a 96 well clean up method that will remove 100 nt/bp and smaller from ~250 bp product? Most give 100 bp as the cutoff, but we've gotten too much small material back with Qiaquick 96. Alternative is Ampure, and it can be tricky to handle in 96 well plate. We'd like to clean up, quantitate, THEN pool and size select to get rid of rest of small material before sequencing. Have tried quantitation, pooling, then clean up and size selection, and dataset yields vary too much. Thanks; Hilary
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