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Old 07-05-2013, 12:30 AM   #1
Location: Europe

Join Date: Dec 2012
Posts: 11
Default Amplicon sequencing - fusion primers ordering

Dear all,

we would like to order fusion primers for 454 GS Junior amplicon sequencing.

When consulting the manual, we are not sure if the primers need to have Phosphorothioated DNA bases on the ends and if B primer needs to be labeled with biotin (or is this true just for shotgun sequencing)?

Thank you for your help.
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