SEQanswers - View Single Post - Amplicon sequencing

MEJ · 07-11-2013, 08:05 AM

Hi,

The phosphorothioate bases are there to inhibit any exonuclease activity, so you can order them without. It did work in our hands but if you get any contamination you'll have a problem!

The biotin on the B primer is necessary for the enrichment (enrichment beads are streptavidin coated magnetic beads) of the amplified beads, regardless the type of library.

07-11-2013, 08:05 AM	#2
MEJ Junior Member Location: Amsterdam Join Date: Dec 2009 Posts: 4	Amplicon sequencing - fusion primers ordering Hi, The phosphorothioate bases are there to inhibit any exonuclease activity, so you can order them without. It did work in our hands but if you get any contamination you'll have a problem! The biotin on the B primer is necessary for the enrichment (enrichment beads are streptavidin coated magnetic beads) of the amplified beads, regardless the type of library.