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Old 07-11-2013, 08:05 AM   #2
MEJ
Junior Member
 
Location: Amsterdam

Join Date: Dec 2009
Posts: 4
Default Amplicon sequencing - fusion primers ordering

Hi,

The phosphorothioate bases are there to inhibit any exonuclease activity, so you can order them without. It did work in our hands but if you get any contamination you'll have a problem!

The biotin on the B primer is necessary for the enrichment (enrichment beads are streptavidin coated magnetic beads) of the amplified beads, regardless the type of library.
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