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Old 07-11-2013, 10:11 AM   #3
ajthomas
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Location: Utah

Join Date: Mar 2010
Posts: 166
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For the FLX, the primers do not need to be biotinylated; standard primers work fine. Biotin doesn't come into the process until the enrichment stage, and the biotin there comes from the enrichment primer, not from the primers you used to create your library. Furthermore, only one molecule of the original library you make with your primers will end up on the bead and even if it was biotinylated, the biotin molecule would be stripped off (along with that original molecule) when you add the melt solution.
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