SEQanswers - View Single Post

krispy · 12-25-2013, 01:10 AM

Thanks for the reply.

I actually got the sequence from the customer letter too (here in: oligonucleotide sequences for the multiplexing sample prep oligo only kit - multiplexing index read sequencing primer).

but may I know is there somewhere stated that this is also the MiSeq Index Primer that comes together with the kit? I wish to synthesize the same primer but load it as custom index primer in different well, I am kinda sure the sequence I got above will work as a custom index primer too even it's not the same with the default one (because it will be run as custom anyway), but I was given a "task" to find out the exact MiSeq default index primer.

12-25-2013, 01:10 AM	#3
krispy Junior Member Location: Singapore Join Date: Apr 2013 Posts: 7	Thanks for the reply. I actually got the sequence from the customer letter too (here in: oligonucleotide sequences for the multiplexing sample prep oligo only kit - multiplexing index read sequencing primer). but may I know is there somewhere stated that this is also the MiSeq Index Primer that comes together with the kit? I wish to synthesize the same primer but load it as custom index primer in different well, I am kinda sure the sequence I got above will work as a custom index primer too even it's not the same with the default one (because it will be run as custom anyway), but I was given a "task" to find out the exact MiSeq default index primer.