I would like to attached more bioanalyzer profiles here. I have selected 3 samples from my library batch as an example.
All 3 samples are proceed in one batch. However, sample A does not have the extra peak beyond 1500bp while samples B and C have!
Does anyone think that the second peak may represent single-stranded library products that have self-annealed? As I don't think it is due to the magnetic bead carryout as I had already aliquot the library on magnetic stand.
Will it affect the sample pooling and do the downstream TruSeq Exome capture?
What can I do to avoid this problem?
Thanks!
Leo
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