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Old 09-22-2014, 07:39 AM   #2
Leo Lee
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Location: HK

Join Date: Apr 2014
Posts: 5
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I would like to attached more bioanalyzer profiles here. I have selected 3 samples from my library batch as an example.

All 3 samples are proceed in one batch. However, sample A does not have the extra peak beyond 1500bp while samples B and C have!

Does anyone think that the second peak may represent single-stranded library products that have self-annealed? As I don't think it is due to the magnetic bead carryout as I had already aliquot the library on magnetic stand.

Will it affect the sample pooling and do the downstream TruSeq Exome capture?

What can I do to avoid this problem?

Thanks!
Leo
Attached Files
File Type: pdf Troubleshoot- extra peak.pdf (249.5 KB, 36 views)
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