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Old 10-13-2016, 10:16 PM   #1
Irene
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Location: Asia

Join Date: Oct 2016
Posts: 2
Default Next-seq starting library concentration for library denaturation

I am following the Nextseq System-denature and dilute libraries guide. The lowest starting library concentration recommended for denature libraries is 0.5 nM. However, the conc. of one of my libraries is particularly low and the final volume would be more than 40 ul.

I would like to know if the volume of the denaturation step is important. If I dilute my libraries at the concentration of 0.25 nM in 80 ul and add 80 ul of 0.2N NaOH followed by 200nM Tris-HCl pH7.0. Would this also work?
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