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Old 09-14-2020, 12:20 AM   #1
frymor
Senior Member
 
Location: Germany

Join Date: May 2010
Posts: 150
Question demultiplex BCL data with two different barcoding systems

Hi,

I was wondering if anyone has experience in demultiplexing BCL files from Illumina with two different barcoding systems in one go?

we would like to know whether it is possible to use different, barcoded Tn5 adaptors during tagmentation and then use those Barcodes in combination with the barcodes on the sequencing primers to demultiplex the samples.

Has anyone has experience with this kind of analysis?
Is it possible to only add the Tn5 sequence to the primer sequence before demultiplexing and use it in the BCL2fastq run?

thanks
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