View Single Post
Old 02-23-2011, 05:51 PM   #2
Location: bay area

Join Date: Mar 2010
Posts: 10

Don't worry about nanodropping your DNA after ChIP. The concentration is most likely <10ng/ul given your conditions so if anything you should be using a Qubit. Just proceed with your favorite chip-seq library prep, using qiagen minelute columns (you can try double-eluting with heated EB, e.g. for a 20 ul elution elute twice with 10 ul hot EB). Do a few test amplifications (15, 18, 21 cycles) and try to re-validate with qPCR on the final library. If that passes and you have sufficient DNA to put on a flowcell, you should be clear.
edawad is offline   Reply With Quote