SEQanswers - View Single Post - "Concave" coverage pattern

ETHANol · 06-10-2011, 08:51 AM

This is a wild guess but I would bet it has to be one of two things: 1) biases generated during PCR amplification of your library. Perhaps the GC content is different near the ends of the chromosomes. Or 2) The ends of the chromosomes fragment more efficiently.

06-10-2011, 08:51 AM	#2
ETHANol Senior Member Location: Western Australia Join Date: Feb 2010 Posts: 308	This is a wild guess but I would bet it has to be one of two things: 1) biases generated during PCR amplification of your library. Perhaps the GC content is different near the ends of the chromosomes. Or 2) The ends of the chromosomes fragment more efficiently.