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Old 11-10-2012, 06:01 PM   #2
Senior Member
Location: Halifax, Nova Scotia

Join Date: Mar 2009
Posts: 381

without knowing what your library prep is....MiSeq is spec'd to sequence 2x250base. So if your total product including adapters etc is below 500 bases you can sequence your product using paired ends and stitch together the over lapping sequences.
you can in theory sequence any size fragment to to the total possible.
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