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JackieBadger · 11-11-2012, 04:22 AM

what modification?
Nextera library prep routinely sequences short fragments.
Just get your PCR product and run it through the TrueSeq library prep and sequence it.
Like I said, there are many ways to prepare a library for sequencing.

11-11-2012, 04:22 AM	#4
JackieBadger Senior Member Location: Halifax, Nova Scotia Join Date: Mar 2009 Posts: 381	what modification? Nextera library prep routinely sequences short fragments. Just get your PCR product and run it through the TrueSeq library prep and sequence it. Like I said, there are many ways to prepare a library for sequencing.