SEQanswers - View Single Post - Library prep woes – unusual bioanalyzer profile of Illumina RNA-seq library

NextGenSeq · 11-19-2012, 10:00 AM

The libraries are okay except the 21 cycle library is over-amplified and beginning to dimerize. If you can see the library on a DNA 1000 chip you have enough to sequence.

11-19-2012, 10:00 AM	#2
NextGenSeq Senior Member Location: USA Join Date: Apr 2009 Posts: 482	The libraries are okay except the 21 cycle library is over-amplified and beginning to dimerize. If you can see the library on a DNA 1000 chip you have enough to sequence.