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Old 11-29-2012, 07:39 AM   #2
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Location: Boston,MA

Join Date: Nov 2008
Posts: 122

I haven't used the NEB chip kit but most kits have a size selection step that would cause other sizes to be removed.

If you skip that step you should get all sizes. When you go to sequence them just remeber to lower the loading concentration a bit as 800 strting will be over 900 bases as a library and takes up quite a bit of real estate on the the flow cell and it is easy to overcluster.
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