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Old 12-05-2012, 06:13 AM   #4
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Location: Belgium

Join Date: May 2009
Posts: 6
Default indexes sequence for multiplexed > 96

We are interested in using dual indexing strategy for multiplexing more than 384 samples in one run on MiSeq instrument. Could we use the 48 indexes (6 bases) provided by Illumina for Truseq Small RNA sample prep as i7 indexes ? how to add 2 bases to these sequences to get unique 48 indexes ? We would use 8 i5 (as Nextera) indexes to reach 384 unique combinations. Do you think this strategy would work? Could we use more than 8 i5 indexes ?
Thans for your help.
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