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Old 11-09-2012, 09:56 AM   #23
Location: United States of America

Join Date: Mar 2011
Posts: 52

Originally Posted by TonyBrooks View Post
Maybe ONT have learnt from PacBio's mistakes. Remember the anticlimax when you first saw PacBio data?

Nanopore error rate is around 4% ~Q14, or at least it was 9 months ago.
The problem is we've all been spoilt by Illumina SBS and will see anything with < Q30 (or even Q20). They've gone on record saying they won't release anything until error is <1% (which is probably nearer the acceptable level). How far away from that they are is anyone's guess at the moment.
PacBio reads, at Q8, are still quite useful for scaffolding assemblies. Crazy to hold back data when there are lots of people who could make use of long Q14 reads right now.
earonesty is offline   Reply With Quote