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Old 04-20-2017, 03:10 AM   #1
ecSeq Bioinformatics
Senior Member
Location: Leipzig, Germany

Join Date: May 2012
Posts: 206
Lightbulb Is there a bias after DNA fragmentation? What do you think?

Cutting DNA into small fragments is a key preparation step for DNA sequencing with NGS technology. To reduce errors and increase reliability of the sequence information, every genomic region should be sequenced several times. This means that several copies of a target DNA have to be cutted in different ways to produce overlapping fragments to ensure an good coverage of the whole region of interest. This approach is based on the general idea that genomic DNA break-points are random and sequence-independent.

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