The Dynabeads protocol calls for a wash buffer that includes Lithium chloride, but other published protocols (ex., http://www.ncbi.nlm.nih.gov/pubmed/21807852) replace LiCl with NaCl. I presume this is because Lithium might inhibit RT, but am not sure. Does anyone who does a lot of Illumina TruSeq-based mRNA-seq have a preferred wash buffer for the oligo-dT beads?
Thanks very much!
Thanks very much!
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