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Old 05-16-2014, 11:08 AM   #8
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Location: London

Join Date: Jun 2009
Posts: 298

Originally Posted by bryanbriney View Post
As of right now, the NextSeq doesn't support dual indexes or custom indexes. Dual indexing is reportedly in the pipeline, not sure about custom indexes. Also, you can't start a run that exceeds the stated capacity of the reagent kit. MiSeq throws an error if you try, but it can be bypassed; NextSeq won't let you continue.
Does this mean you can't do more than 75 cycles in a 75 cycle kit. I confirmed with tech support that there are 25 additional cycles for dual indexing and we were told we could use those 100 cycles as we wished. We were hoping to do 47|6|47 from the 75 cycle kit
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