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Old 05-24-2019, 12:04 PM   #8
Location: Atlanta

Join Date: Nov 2018
Posts: 11

The only two things I can think of is DNA in buffers containing high concentrations of EDTA, other chelating agents or salts may inhibit the
end repair and A-tailing reaction. The other is something going wrong with library amplification (maybe your index primers).

If you can, check the concentration or run a gel/tape of the adapter-ligated library to see if the yield is reasonable going into library amplification.
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