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Old 11-15-2013, 01:57 AM   #1
avo
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Location: Germany

Join Date: Sep 2013
Posts: 14
Default TruSeq PCR-free transition

Hi together,

my lab is planning to switch to the TruSeq PCR free library prep. We would like to perform size selection on a bluepippin. Reading various threads here about the low yields of the protocol i am wondering if the replacement of the bead based size selection by a pippin might result in to low yields to meet the cluster densities possible with the new V3 reagent kits?

Does anyone have experience with this matter yet?

Thanks

Last edited by avo; 11-15-2013 at 04:13 AM.
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