Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Sure, you can still pick up chromosomal and other structural rearrangements with exome sequencing, just not 100% of the time. You're correct that the reason this worked was due to the genes rearranging and effectively merging in the genome. Because of that, the capture kit still got reads that mapped to the end of one or the other of the genes (with the remainder mappable to the other gene).
This would be in contrast to detecting fusion transcripts arising from neighboring genes being coexpressed and spliced together. Those sorts of situations wouldn't be detectable with exome sequencing.
-
The answer is that it depends on:
- where fusion junction point lies (it is "well" inside an exon which should be 'captured' properly and both genes involved in fusion should captured properly),
- coverage of sequencing
Whole genome sequencing and RNA-seq are ones of the most used for finding fusion genes and/or translocations.
Comment
-
For anyone interested: They used dRanger (not publicly available) and Breakpointer in order to detect the fusion from exome data.
Comment
Latest Articles
Collapse
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
25 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
28 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
52 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment