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Old 04-03-2013, 09:02 AM   #3
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317
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Quote:
Originally Posted by josdegraaf View Post
Illumina recommended us to do a maintenance wash before the run using the same gaskets as during the real run..
How does that work for you?

By the way, I follow exactly that "gasket change/maintenance wash" protocol. I am not sure what problem that is trying to solve -- maybe it removes any solvents that might leach from the gaskets?

However it does not solve the issue I am talking about here. These are not bubbles coming from the gasket seal, they are present in the lines leading to the seals. You can see the bubbles coming through the tubes drilled through the transparent amber plastic blocks leading to the seals. Although you might need a magnifying glass and a light source to see them.

I think these bubbles are from gas dissolved in the scanning buffer coming out of solution during pumping. That is my guess anyway.

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Phillip
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