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Old 04-03-2013, 10:56 AM   #4
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Location: East Coast USA

Join Date: Feb 2008
Posts: 7,015

Are there temperature fluctuations (day/night cycles or otherwise) in the room where your HiSeq resides?

I remember bubbles being a issue in the past when we had problems with temperature control (though I can't definitely say they were related, just anecdotal thinking). Lately I have not heard of bubbles being a problem.
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