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Old 04-04-2013, 05:32 AM   #7
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317
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Quote:
Originally Posted by GenoMax View Post
I had a look at a couple of the recent HiSeq (2000 and 2500) flowcells (following your path above). I checked a few cycles and high(er) error tiles. Did not see any bubbles on tiles I checked.

I am not on experimental side of things but keep an ear open for problems like this since they ultimately affect data.
Did you see the big difference between the top and bottom surface on the error rate plot?

We only have one instrument, so I don't know if it is normal to have that number of bubbles.

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Phillip
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