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Old 07-08-2013, 01:20 PM   #1
Location: New Jersey

Join Date: Jun 2013
Posts: 22
Angry Failed tagmentation reaction for Nextera library?

Just had failures #2 and #3 using Illumina Nextera. I was able to run a sample from failure #1 of the post-zymo cleanup on Agilent 2100 Bioanalyzer this morning, and it looked almost identical to the input genomic DNA. Haven't run the samples from todays failures, but suspect they would look similar. This points to a failed tagmentation reaction. Anyone else ever experience this? If so, is it due to bad enzyme, or a problem with your input DNA? FYI, the 3 samples came from 2 different labs/ 2 different species. #2 was a re-precipitate of #1 to remove any potential inhibitors present in the resuspension buffer. I'd really appreciate any input you might have!
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