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Old 08-05-2013, 01:25 PM   #3
Location: New Jersey

Join Date: Jun 2013
Posts: 22

I finally had a chance to run 3 different clean-up protocols and make Nextera with the resulting DNA. I used Qiagen plant DNeasy miniprep, MoBio Power Clean DNA Clean-Up Kit, and Zymo Research OneStep PCR Inhibitor Removal Kit. The only one that gave a result was the MoBio Power Clean. I finally had a detectable (by Qubit) concentration of DNA in my library, and on the Bioanalyzer I finally see fragments 300bp to >1kb. All others I still only see some remnant genomic input on the Bioanalyzer. THANK YOU to ZWB for your suggestion, now I can move forward with this library after a month of pulling out my hair and many apologies and explanations to our collaborator. Hooray!
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