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Old 02-20-2014, 05:05 PM   #7
lbcbci
Junior Member
 
Location: California

Join Date: Aug 2011
Posts: 5
Default Nextera DNA sample kit protocol

I am recently using Nextera DNA sample kit for library prep. According user guide, the spin speed was set as 1300xg for PCR clean up with Zymo column. Actually there is only about 4000rpm (Eppendorff centrifuge) and the column looks wet still after spin. Is anybody aware about this? Does it better to increase spin speed?

Appreciate your sharing!

LBC
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