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Old 04-07-2016, 05:46 PM   #11
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,230
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I think the only difference is concentration of enzymes. Following to consider:
1- The buffer used to elute from columns. If TE has been used, inhibition of tagmentation enzymes is expected.
2- If you have enough DNA you can do another clean up with 1.2x AMPure bead or a column and elute in TE0.1
3- GC content of genome, normally %GC 30-75 is OK
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