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Old 09-15-2010, 12:45 AM   #1
Junior Member
Location: cambridge

Join Date: Aug 2010
Posts: 6
Default problem with mRNA multiplex libraries

I have been trying to generate mRNA-SEQ multiplex libraries using the multiplex kit from Illumina but my end repair, dA-tailing and ligation reagents are from NEB labs. I have no problem generating a smear post PCR if I use the standard Illumina PE reagents but do not see any PCR smears using the multiplex kit. I cannot see what is the difference in the protocol except using different adapters and primers. Can anyone help please? Thanks
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