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Old 11-17-2011, 12:43 PM   #5
HESmith
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Location: Bethesda MD

Join Date: Oct 2009
Posts: 505
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Simcom: Judicious primer design coupled with the appropriate annealing temperature will virtually assure that the primer does not hybridize inappropriately.

Genomax: the designation of a normal complexity sample as the control lane does not solve the problem (sadly). While it allows the signal thresholds to be set appropriately, it doesn't address the problem of cluster resolution in the low complexity lanes.
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