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Old 11-17-2011, 12:50 PM   #6
Simcom
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Location: East Coast US

Join Date: Jun 2010
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Quote:
Originally Posted by GenoMax View Post
Can your core not run your samples by specifying a different lane (which is expected to have "normal" DNA) as the "control" lane for that run?
I think you are misunderstanding the problem. The issue is, because the first 5nt of read #1 are going to be all identical among clusters, and the machine uses these 5nt to call clusters, the machine has a hard time identifying/differentiating between different clusters (especially close overlapping clusters). So the reason for the gDNA is to add diversity to the sequence, allowing clusters to be called. Hence the reason it needs to be included in the same lane as the sample.
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