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Old 11-17-2011, 12:59 PM   #8
Simcom
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Location: East Coast US

Join Date: Jun 2010
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Quote:
Originally Posted by HESmith View Post
Simcom: Judicious primer design coupled with the appropriate annealing temperature will virtually assure that the primer does not hybridize inappropriately.
I agree with you that hybridization is unlikely, but if it does happen it will be indistinguishable from an actual integration. Among other things, we are interested in mapping low-abundance integrations, so if we aren't able to get the verification sequence on every read, we will likely need to go in and verify a subset of integrations manually, which may not be possible if an integration is present in only one cell for example.
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