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Old 11-17-2011, 01:11 PM   #11
Simcom
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Location: East Coast US

Join Date: Jun 2010
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Quote:
Originally Posted by HESmith View Post
If, as you say, you can tolerate discarding 90-95% of the reads, then spiking in a gDNA library at that level will definitely solve your problem. After all, adapter dimers are often present at 5-10% in many libraries (the same % as your desired samples), and sequencing them is not a problem!
Thanks, this gives me confidence. Just to be sure though: do the adapter-adapter ligation reads come back in the data, or does the machine throw them out and not include them in sequencing results? If you actually get the adapter-adapter reads from the machine, I should be golden.
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