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Old 11-17-2011, 02:00 PM   #15
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Location: East Coast US

Join Date: Jun 2010
Posts: 8

Originally Posted by pmiguel View Post
We got it to work on our HiScanSQ -- which uses the same chemistry as the HiSeq, but only scans the top of the flowcell. Not an identical situation, but we had some SMART cDNAs that we sheared and ligated TruSeq adapters on. So about 1/2 of them had the same 50 nt of SMART primer at the beginning. We mixed them 1:1 with a genomic DNA library. Cluster registration went fine.

OK, that is good to hear. I'm not sure why my core was having trouble spiking 1:1 gDNA.
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