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Old 11-18-2011, 04:59 AM   #18
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317

I have seen bad batches of phiX that had fairly high (a few percent, I think) adapter dimers levels in them. Maybe you should make your own genomic DNA library to make sure your "diluent" is of high quality.

Also you could obtain your "diluent" by sub-contracting a sequencing job. Send an email out to a prospective department (maybe one with a high level of plant or fungal sciences being done) and offer a one time only discount genome sequence. Our diluent was a sorghum genomic DNA library.

pmiguel is offline   Reply With Quote