Hi all,
I was wondering whether anyone has come across a column-based cleanup kit, which would remove large primers (EMP 16S design) and primer-dimers of around 100bp. Or alternatively, protocols to "abuse" silica columns to behave accordingly.
I'm running a course with students, so I am pretty sure the budget won't stretch for beads and magnetic plates. Unfortunately, given my number of students and time-constraint, I cannot always do the right thing and get the PCR optimised properly - I'm happy it works most of the time .
I've also come across a sample recently (raw milk), where all my troubleshooting wouldn't get rid of the pesky primer-dimers. So I can appreciate that this would happen to one of my flock.
Thanks!
Mike
I was wondering whether anyone has come across a column-based cleanup kit, which would remove large primers (EMP 16S design) and primer-dimers of around 100bp. Or alternatively, protocols to "abuse" silica columns to behave accordingly.
I'm running a course with students, so I am pretty sure the budget won't stretch for beads and magnetic plates. Unfortunately, given my number of students and time-constraint, I cannot always do the right thing and get the PCR optimised properly - I'm happy it works most of the time .
I've also come across a sample recently (raw milk), where all my troubleshooting wouldn't get rid of the pesky primer-dimers. So I can appreciate that this would happen to one of my flock.
Thanks!
Mike
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