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Old 04-19-2018, 06:46 AM   #3
Pauline dlp
Junior Member
Location: Philippines

Join Date: Mar 2018
Posts: 7

Thanks for the reply, nucacidhunter! So it seems methylation sensitivity is more of an issue for plants. I tried doing in silico digests of my reference genome with several REs, and the methylation insensitive ones give a decent number of fragments for the target size (~100-200 bp).

We do have a PippinPrep, would bring up the possibility of using ddRAD. But i'm not sure I could do that since the project proposal stated GBS methods will be used.
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