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Old 05-27-2019, 02:24 AM   #2
Location: Brazil

Join Date: Aug 2015
Posts: 92

The confusion comes from the use of "mismatch" both for "sequencing error" and "difference between barcodes".
If you allow one sequencing error, then the number of differences between barcodes must be equal to (2*sequencing errors + 1) = 3. Otherwise, for example, if the sequencer reads CACCTGAT it won't be able to attribute this to the first barcode with one sequencing error, or the second barcode with one sequencing error.

In your case, you should allow zero mismatches (sequencing errors) due to having barcodes with two mismatches (differences).

Last edited by r.rosati; 05-27-2019 at 02:32 AM.
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