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Old 05-27-2019, 03:51 AM   #4
Location: Brazil

Join Date: Aug 2015
Posts: 92

Glad to be of help!
As an afterthought - one can't blame the software for calling both "mismatches". Not the sequencer, nor the software know the "truth" and they don't know if a base is a sequencing error or not. So for the software, a mismatch is a mismatch; if it was known that a called base was an error, it wouldn't have been called. Perhaps I should have phrased the two as (1) "mismatch between the read sequences vs the barcode sequences" and (2) "mismatch between the two expected barcodes".

Last edited by r.rosati; 05-27-2019 at 05:29 AM.
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