Hi Everyone:
I am doing exome sequencing and I just had a basic question about read trimming. Is read trimming at the 3' end AND/OR by base quality score completely necessary? What influence will NOT trimming have on downstream variant calling? I was thinking that low quality bases would be accounted for by their low base qualities and thus we would not have to completely omit or "trim" these bases?
Thanks,
MC
I am doing exome sequencing and I just had a basic question about read trimming. Is read trimming at the 3' end AND/OR by base quality score completely necessary? What influence will NOT trimming have on downstream variant calling? I was thinking that low quality bases would be accounted for by their low base qualities and thus we would not have to completely omit or "trim" these bases?
Thanks,
MC
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