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Old 02-15-2018, 11:27 AM   #5
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,300

Well, one thing. You mentioned you changed the sample sheet after the run. What sample sheet was used by the MiSeq during the run?

The length of the i7 index read is determined by the length of the sequences in the sample sheet. So if you did not have 12 base indexes in the run sample sheet, then that index read would be whatever length the indexes you did use were. And, if you didn't provide any indexes, then no index read would have been done and it would likely be impossible to demultiplex you run.

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