Hi! I am trying to find the fastest sequencing machine. How long does the sequencing take at minimum? Does the bridge amplification, sequencing, or data processing take most time? Why does the sequencing take time, if there is only 250 bases to sequence (as all reads are read simultaneously)? Thanks in advance for the comments!
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Minimum run time on Illumina systems is 4 hours on MiniSeq and MiSeq for 36 cycles. Generally, sequencing takes long time. In every cycles depending on 1, 2 or 4 colour chemistry few steps is required which includes base incorporation, imaging, debolcking and washing. YouTube search should find videos on different Illumina chemistries. One colour chemistry have slightly different workflow.
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Originally posted by Kultamuna2 View PostHi! I am trying to find the fastest sequencing machine. How long does the sequencing take at minimum? Does the bridge amplification, sequencing, or data processing take most time? Why does the sequencing take time, if there is only 250 bases to sequence (as all reads are read simultaneously)? Thanks in advance for the comments!Last edited by snetmcom; 03-07-2018, 10:27 PM.
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Originally posted by nucacidhunter View PostMinimum run time on Illumina systems is 4 hours on MiniSeq and MiSeq for 36 cycles. Generally, sequencing takes long time. In every cycles depending on 1, 2 or 4 colour chemistry few steps is required which includes base incorporation, imaging, debolcking and washing. YouTube search should find videos on different Illumina chemistries. One colour chemistry have slightly different workflow.
I have also heard that read length affects the sequencing time: shorter the read length, faster the sequencing. Why isn't then all sequencing carried out with this 36bp read length? Do we get lower quality data with lower read length?Last edited by Kultamuna2; 03-12-2018, 03:38 PM.
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