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Old 03-15-2018, 02:07 PM   #10
athuyvo
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Location: USA

Join Date: Jul 2017
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Thanks @thermophile. I have same exact format for my sample sheet. I was able to requeue the analysis by essentially ignoring the i7 reads and demultiplex using only i5 barcodes. We think the problem was suboptimal custom i7 primers so the MiSeq couldn't demultiplex using i7 reads.
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