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Old 11-09-2015, 02:07 AM   #9
frymor
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Location: Germany

Join Date: May 2010
Posts: 149
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Quote:
Originally Posted by dpryan View Post
BTW, I hope "replica" doesn't denote the biological replicates
Now I am confused. "replica" are the biological replica I have.

This is my colData:
Code:
sampleName	hours	replica	batch
IFM_Myoblast_1	0	1	2014
IFM_Myoblast_2	0	2	2014
IFM_Myoblast_3	0	3	2014
IFM16h_1	16	1	2013
IFM16h_2	16	2	2015
IFM16h_3	16	3	2015
IFM24h_1	24	1	2013
IFM24h_2	24	2	2015
IFM24h_3	24	3	2015
IFM30h_1	30	1	2013
IFM30h_2	30	2	2014
IFM48h_1	48	1	2013
IFM48h_2	48	2	2014
IFM48h_3	48	3	2014
IFM72h_1	72	1	2013
IFM72h_2	72	2	2014
IFM90h_1	90	1	2013
IFM90h_2	90	2	2013
IFM100h_1	100	1	2013
IFM100h_2	100	2	2014
As you can see, replica are the biological replica. I am assuming that thee replica are not significantly different from each other and therefore would like to assume, that within each time-point, I don't have any significant differences.

BUT

If i do have them there, I would like them to be ignored (=not taken into account, when calculating the differential expression). For that reason I do like to add them to the reduced model, if I understood it correctly.
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