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Old 01-07-2016, 09:12 AM   #7
Rick Westerman
Location: Purdue University, Indiana, USA

Join Date: Jun 2008
Posts: 1,104

Originally Posted by GenoMax View Post
@Alex852013: A large % of reads is still failing to align (if you are only getting 23% of reads to align) so you may want to investigate why that is so. Quick blasting @NCBI should give a clue as to whether you data has unexpected contamination.
Didn't he say 23% more not 23% total? In any case the original post talked about mapping a mixture of plasmid and E.coli reads against the plasmid and thus he expected a low percentage.

Last edited by westerman; 01-07-2016 at 09:13 AM. Reason: Added question mark.
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