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Old 07-17-2018, 05:11 AM   #3
emily202
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Location: FL

Join Date: Sep 2012
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Thanks for your reply.

Yes, RNA-seq can provide what's transcriptionally active at the time of sampling. I'm wondering if there is an RNA workflow similar to 16S metagenomic sequencing for taxonomic classification. Perhaps this is more of an analysis tool question than methodology, however, I'm proposing total RNA isolation, host depletion and bacterial enrichment prior to library generation since these samples are suspected to be of low bacterial load.

Does anyone have experience or suggestions for bacterial taxonomic characterization using RNA-seq?
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