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Old 03-25-2019, 12:48 AM   #1
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Location: HK

Join Date: Mar 2019
Posts: 2
Default Newbie Help for 1D^2 library shearing

Hi, all!
I've done my 1st trial of 1D^2 whole genome sequencing using LSK-SQK308 kit with R9.5 flowcell.
Without doing library fragmentation, I encountered the unexpected library shearing during sequencing (see, which wasn't seen in the QC gel photo before (see and after library preparation (see The bands had no significant low molecular weight fragments.
Anyone using nanopore has encountered this before? Any explanation? And how to avoid it? Since I could not find the problem in my QC steps...


Last edited by yiyi_Z; 03-25-2019 at 12:51 AM.
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