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Old 03-25-2019, 12:48 AM   #1
yiyi_Z
Junior Member
 
Location: HK

Join Date: Mar 2019
Posts: 2
Default Newbie Help for 1D^2 library shearing

Hi, all!
I've done my 1st trial of 1D^2 whole genome sequencing using LSK-SQK308 kit with R9.5 flowcell.
Without doing library fragmentation, I encountered the unexpected library shearing during sequencing (see https://imgur.com/O7PVgF3), which wasn't seen in the QC gel photo before (see https://imgur.com/xrnX09v) and after library preparation (see https://imgur.com/P1pTC6e). The bands had no significant low molecular weight fragments.
Anyone using nanopore has encountered this before? Any explanation? And how to avoid it? Since I could not find the problem in my QC steps...

Thanks!
Yiyi

Last edited by yiyi_Z; 03-25-2019 at 12:51 AM.
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