View Single Post
Old 04-01-2019, 03:28 AM   #2
Bukowski
Senior Member
 
Location: UK

Join Date: Jan 2010
Posts: 390
Default

People have been doing similar things on the HiSeq too - but the issue here isn't whether it's technically feasible to hack the kits, it's whether your application will be able to cope with the awful sequencing quality of anything over 300bp in length. If you routinely use the 600 cycle kits, you'll know how the R2 drop off is - so imagine what this is going to be like after 700 cycles...

But see also: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3673215/
Bukowski is offline   Reply With Quote