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Old 04-02-2019, 06:31 AM   #4
Femta
Junior Member
 
Location: Germany

Join Date: Oct 2018
Posts: 4
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Hi,

well you initially asked why there are these small fragments ;-) And since you didn't see them in your QC before the library prep it's most likely that that happen due to pipetting during the library prep.

But yes, the adapter ligation step is the most critical step during this protocol. You might also loose more than you expect, so it can be helpful to increase the DNA input in advance.
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